Test Code IMFX Immunofixation, Serum
Specimen Required
Only orderable as part of a profile. For more information see MPSS / Monoclonal Protein Study, Serum.
Patient Preparation: Fasting preferred but not required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
800306Useful For
Aids in diagnosis of monoclonal gammopathies when used in conjunction with urine monoclonal studies
Identification and isotyping of monoclonal immunoglobulin heavy and light chains
Documentation of complete response to therapy
Method Name
Only orderable as part of a profile. For more information see MPSS / Monoclonal Protein Study, Serum.
Immunofixation
Reporting Name
ImmunofixationSpecimen Type
SerumSpecimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum | Refrigerated (preferred) | 28 days |
Frozen | 28 days | |
Ambient | 14 days |
Reference Values
Only orderable as part of a profile. For more information see MPSS / Monoclonal Protein Study, Serum.
Immunofixation: No monoclonal protein detected
Flag, Immunofixation: Negative
Performing Laboratory

Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86334
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
IMFX | Immunofixation | 74665-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
81653 | Immunofixation | 74665-1 |
606977 | Flag, Immunofixation | No LOINC Needed |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Other | 3 freeze/thaw cycles |
Day(s) Performed
Monday through Friday
Report Available
3 to 7 daysReflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IFXED | Immunofixation Delta and Epsilon, S | Yes | No |
Testing Algorithm
If a monoclonal light chain is detected in the absence of an associated monoclonal heavy chain, immunofixation electrophoresis specific for delta and epsilon chains is performed, if not previously performed, or at the discretion of the laboratory director reviewing the case.