Test Code IMFXO Immunofixation Only, Serum
Specimen Required
Patient Preparation: Fasting preferred but not required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Identification of monoclonal immunoglobulin heavy and light chains
Documentation of complete response to therapy
Method Name
Immunofixation
Reporting Name
Immunofixation Only, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum | Refrigerated (preferred) | 28 days |
Frozen | 28 days | |
Ambient | 14 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Immunofixation: No monoclonal protein detected
Immunofixation Flag: Negative
Report Available
2 to 7 daysPerforming Laboratory

Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86334
86334-Immunofixation Delta and Epsilon (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
IMFXO | Immunofixation Only, S | 74665-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
32436 | Immunofixation | 74665-1 |
606978 | Flag, Immunofixation | No LOINC Needed |
Secondary ID
800316Day(s) Performed
Monday through Friday
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IFXED | Immunofixation Delta and Epsilon, S | Yes | No |
Testing Algorithm
If a monoclonal light-chain is detected in the absence of an associated monoclonal heavy-chain, immunofixation electrophoresis specific for delta and epsilon chains is performed, if not previously performed, or at the discretion of the laboratory director reviewing the case.