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HelpTest Code MDNS Methadone and Metabolites, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.0 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Useful For
Compliance monitoring of methadone
Assessment of methadone toxicity
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Methadone and Metabolite, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Methadone:
Therapeutic: 100-400 ng/mL
Toxic: >2000 ng/mL
EDDP:
Not established
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Methadone: 10 ng/mL
EDDP: 5.0 ng/mL
Day(s) Performed
Tuesday, Thursday
Report Available
3 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
G0480
80358 (if appropriate for select payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MDNS | Methadone and Metabolite, S | 96602-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36309 | Methadone | 3772-1 |
| 36310 | EDDP | 60071-8 |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.